pyrus × bretschneideri

Transcriptomic and Functional Analyses Reveal That PpGLK1 Regulates Chloroplast Development in Peach (Prunus persica). Ka/Ks >1). Each of these BZR genes from the five Rosaceae species contributed at least one BZR gene to these four groups, implying that the BZR gene family presents a conservative evolutionary trend in Rosaceae species. (2018). The previously published studies have proven that the BZR protein has a highly conserved N-terminal domain with DNA-binding activity in vitro and in vivo (He et al., 2005; Yin et al., 2005). The chromosomal-level sequences of both the European (Pyrus communis) and Chinese (P. bretschneideri) pear genomes have not been published in public databases so far. Pyrus bretschneideri (Pear). The fluorescence signal of 35S:GFP was found to distribute throughout the cell, whereas the fluorescence signals of the GFP fusion proteins were specifically found to be localized in the nucleus (Figure 5A), which was confirmed by DAPI staining. The yellow fruits are eaten in China, where there are selections with ‘excellent fruit’ (Lu et al. Pyrus x bretschneideri: Taxonomy navigation › Pyrus. In P. communis, two BZR gene members were found on chromosome 10 and 15, and one member on chromosome 5, 6, 7, 8, 13, and 17, and remaining gene members located different scaffolds. Figure 4 Sliding window plots of duplicated BZR genes in Pyrus bretschneideri and Pyrus communis, respectively. 127:262-270. DIRs are found in almost all vascular plants (Davin and Lewis, 2000), which often in the form of the gene family. Pyrus bretschneideri commonly known as White pear, ya pear or pearple or Chinese white pear, is an interspecific hybrid species of pear native to North China, where it is widely grown for its edible fruit. The relative expression levels were summaried based on the 2−ΔΔct method (Livak and Schmittgen, 2001). 17, 161. doi: 10.3390/ijms17020161, He, J. X., Gendron, J. M., Yang, Y., Li, J., Wang, Z. Y. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). A-D). Genetic relationships of Pyrus species and cultivars native to East Asia revealed by randomly amplified polymorphic DNA markers. 23, 396–408. Taxonomic note Jacobson (1996) considers P. bretschneideri to be a hybrid between P. pyrifolia and P. betulifolia. The previously published articles showed that PEST domains are contributed to controlling protein stability (Yin et al., 2005). Cell 109, 181–191. reported.! (2014). (2013) found that the BZR gene controlled the size and shape of seeds by regulating the expression of genes such as MINISEED3 and HAIKU2. Roots, stems, leaves, and fruits were collected and frozen immediately in liquid nitrogen and stored at −80 °C. doi: 10.1126/science.1153917, Tang, W., Yuan, M., Wang, R., Yang, Y., Wang, C., Osesprieto, J. Pyrus bretschneideri is a deciduous Tree growing to 6 m (19ft 8in). doi: 10.1093/molbev/msr121, Tang, H., Bowers, J. E., Wang, X., Ming, R., Alam, M., Paterson, A. H. (2008). Una ning gihulagway ni Alfred Rehder. Polyphenol oxidase (PPO) was isolated from Yali pear (Pyrus bretschneideri R). Figure S6 | Figure S6 qRT-PCR verification of 10 pear genes in seven developmental stages of pear fruits, including 15 days after flower (DAF), 30DAF, 55DAF, 85DAF, 115DAF,130DAF and 145 DAF. Mol. Our data presented that these PbBZR genes contained similar expression patterns [Pearson correlation coefficient (PCC) > 0.7] as RNA-seq (Figure S6). Under photoperiodic control, seedlings were grown in incubator for 40 days. However, no systematic, in-depth study of the BZR gene family has been reported in five Rosaceae species, including F. vesca, P. mume, P. persica, P. communis, and P. bretschneideri. Hortic. Front. Genetic relationships of Pyrus species and cultivars native to East Asia revealed by randomly amplified polymorphic DNA markers. 2! A., Maere, S., Van De Peer, Y. Prunus_persica Detail . Methods 25, 402–408. Pyrus × bretschneideri. Jiang et al. Trends Plant Sci. At the beginning of the microsynteny analysis, we prepared three property files (i.e. Mol. All materials used in this study were collected from pear trees (Pyrus bretschneideri Rehd. gene identifier file, gene list file and the CDS file) based on the previously published manuscripts (Cao et al., 2016a; Cao et al., 2016d). 8, 806. doi: 10.3389/fpls.2017.00806, Saha, G., Park, J., Jung, H., Ahmed, N. U., Kayum, M. A., Kang, J., et al. Interaction of PbBZR1 protein with promoters of PbHCT6, PbCES9, PbCCoAMOT1, PbCOMT3, and PbCCR20 was further confirmed by a transient transcription assay. Bot. Sci. Huang LJ, Gao WY, Li X, Zhao WS, Huang LQ, Liu CX (2010) Evaluation of the in vivo anti-inflammatory effects of extracts from Pyrus bretschneideri Rehd. Sci. Pear (Pyrus), as the third-most crucial temperate fruit crop, has been fully sequenced. Datasheet Type(s): Host Plant, Exclude from ISC. To further understand the putative function of all PbBZR genes, we examined their expression patterns in four tested tissues, including roots, stems, leaves and fruits in P. bretschneideri. 白梨 bai li Trees to 5–8 m tall. In contrast, the BZR gene family members of the other three Rosaceae species have experienced an ancient whole duplication event and followed by gene loss events, which ultimately resulted in fewer members of the BZR gene family than P. communis and P. bretschneideri. Acad. Amer. Right panel: distribution of conserved motifs and exon-intron structure. Pyrus bretschneideri là loài thực vật có hoa trong họ Hoa hồng. The data showed that the expression of different PbBZRs varied between roots, stems, leaves and fruits, and some of them were preferentially expressed in specific tissues (Figure S5). The Primer-BLAST was used to design gene-specific primers for cloning and qRT-PCR. Free and Open Access to Biodiversity Data. 2003). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. Table 1 Duplicated BZR gene pairs in Pyrus bretschneideri and Pyrus communis. (2013). Biol. Synteny and collinearity in plant genomes. org/ articles/ pyrus/ pyrus-bretschneideri/). doi: 10.1093/molbev/msu300, Oh, E., Zhu, J., Wang, Z. Pyrus bretschneideri is a perennial woody deciduous member of the Pyrus genus in the family Rosaceae. Figure 3 Scatter plots of the Ka/Ks ratios of paralogous BZR genes in pear and orthologous BZR genes among five Rosaceae genomes. Proceedings of the American Academy of Arts and Sciences. Each colony were subsequently incubated overnight at 28°C in 1 mL LB medium having 10 mM MES, 20 mM acetosyringone, 50 mgmL−1 rifampicin, 50 mg mL−1 gentamicin and 50 mg mL−1 kanamycin. Pyrus bretschneideri Rehder is an accepted name This name is the accepted name of a species in the genus Pyrus (family Rosaceae). The TBtools software was used to visualize gene structure and chromosomes positions of BZR genes in these Rosaceae genomes based on the GFF annotation files (Chen et al., 2018a). Han, Y., Ding, T., Su, B., Jiang, H. (2016). The conserved motif and multiple sequence alignments analyses showed that most of the BZR proteins contain a typical bHLH domain (basic helix–loop–helix) in these sequences N-termini represented by motif 1 (Figure S4). The record derives from Tropicos which reports it as an accepted name (record 27802404) with original publication details: Proc. Taxonomy. Plant Biotechnol. Fragaria_vesca Detail . Front. Recommended citation 'Pyrus bretschneideri' from the website Trees and Shrubs Online (treesandshrubsonline. Int. 11:1087. doi: 10.3389/fpls.2020.01087. 7, 577. doi: 10.3389/fpls.2016.00577, Cao, Y., Han, Y., Li, D., Lin, Y., Cai, Y. Chromosomal level reference genomes provide a crucial foundation for genomics research such as genome-wide association studies (GWAS) and whole genome selection. Acad. Nature 422, 433–438. Guo, Y. F., Shan, W., Liang, S. M., Wu, C. J., Wei, W., Chen, J. Y., et al. At the end of purification by ion exchange chromatography on DEAE‐cellulose, 10.8‐fold purification was achieved. MCScanX: a toolkit for detection and evolutionary analysis of gene synteny and collinearity. doi: 10.1038/nature01521, Cai, B., Yang, X., Tuskan, G. A., Cheng, Z.-M. (2011). Topic. It is hardy to zone (UK) 5. The SPSS 20.0 was used to perform the statistical analyses. Genome-Wide Identification, Characterization and Expression Analysis of the Chalcone Synthase Family in Maize. In our study, the 55-days fruits were selected because the lignin content of the P. bretschneideri fruit peaked during this period. General Information Pyrus bretschneideri is a deciduous tree that can grow 5 - … Weighted Gene Co-Expression Network Analysis (WGCNA) showed that the PbBZRs are in the same module as the key genes of the lignin metabolism pathway, such as PbCCoAMOT and PbCCR, indicating that they may be involved in regulating lignin biosynthesis (Zhang et al., 2016; Cao et al., 2019). The effector was PbBZR1, which was driven by the 35S promoter (Figure 7B). (2012). Fruit_stage1, Fruit_stage2, Fruit_stage3, Fruit_stage4, Fruit_stage5, Fruit_stage6 and Fruit_stage7 indicated 15 days after flower (DAF), 30 DAF, 55 DAF, 85 DAF, 115 DAF, 130 DAF and145 DAF, respectively. Clades of BZR genes (1–4) are highlighted with different colored backgrounds. Pyrus bretschneideri Rehder, Proc. 5 Mol. Physiol. According to the expression analysis of the PbBZRs during fruit development, three PbBZRs were selected for detailed analysis. Cross-reference K74. The enzyme showed activity to catechol, pyrogallol, chlorogenic acid and DL‐DOPA; of these four, chlorogenic acid was the best substrate. The material of plant was ‘Dangshansuli’, cultivar variety is Pyrus bretschneideri Rehd, which developing at Nanjing region, China. Nucleic Acids Res. were selected as materials from a commercial orchard in Dali County, Shaanxi Province, China, in 2014 and 2015. Group C contains the fewest BZR genes (five), while group A has the most BZR genes (seventeen), followed by group B (fourteen) and group D (eleven). A dual luciferase reporter assay was used in plant cells. Figure 8 PbBZR1 inhibits the transcription of PbHCT6, PbCES9, PbCCoAOMT1 and PbCCR20. doi: 10.1186/gb-2013-14-4-r36, Letunic, I., Doerks, T., Bork, P. (2012). Figure 6 VIGS technique applied to the Pyrus bretschneideri fruits. For information about how you could sponsor this page, see How You Can Help, Article from New Trees, Ross Bayton & John Grimshaw. doi: 10.1073/pnas.152342599, He, J., Gendron, J. M., Sun, Y., Gampala, S. S., Gendron, N., Sun, C. Q., et al. The previously published manuscripts have confirmed that the BZR genes could regulate plant architecture, development of fruit, spikelet and root, and promote cell elongation (Clouse et al., 1996; Li and Chory, 1997; Ye et al., 2010; Clouse, 2011). We are good to grow: dynamic integration of cell wall architecture with the machinery of growth. P. bretschneideri fruit developmental stage 1 (15DAF), Accession: SRX1595645; P. bretschneiderifruit developmental stage 2 (30DAF), Accession: SRX1595646; P. bretschneiderifruit developmental stage 3 (55DAF), Accession: SRX1595647; P. bretschneideri fruit developmental stage 4 (85 DAF), Accession: SRX1595648; P. bretschneiderifruit developmental stage 5 (115 DAF), Accession: SRX1595650; P. bretschneiderifruit developmental stage 6 (130 DAF), Accession: SRX1595651; P. bretschneiderifruit developmental stage 7 (145 DAF), Accession: SRX1595652; P. bretschneideri leaves_1, Accession: SRR5866154; P.bretschneiderileaves_2, Accession: SRR5866157; P. bretschneideri leaves_3, Accession : SRR5866160; P. bretschneideri roots_1, Accession: SRR5866155; P. bretschneideri roots_2,Accession: SRR5866158; P. bretschneideri roots_2, Accession: SRR5866161; P. bretschneideristem_1, Accession: SRR5866156; P. bretschneideri stem_2, Accession: SRR5866159; P.bretschneideri stem_3, Accession: SRR5866162; P. bretschneiderifruit_1, Accession : SRR4492447; P. bretschneiderifruit_2, Accession: SRR4492450.The authors affirm that all data necessary for confirming the conclusions of the article are present within the article, figures, tables and Supplementary Materials. Acad. At the end of purification by ion exchange chromatography on DEAE‐cellulose, 10.8‐fold purification was achieved. (2002). Pyrus x bretschneideri Taxonomy ID: 225117 (for references in articles please use NCBI:txid225117) current name Next, all the 47 BZR proteins from these five species were subjected to the online MEME website to detect conserved motifs shared among related proteins (Figure S3). Black triangle represented BZR genes, and same color indicated these genes in the same fragment. (2007) classified the … These trees were divided into four groups, designated as A, B, C and D. Figure 2 Interspecies microsynteny of BZR genes in five Rosaceae genomes, including Fragaria vesca, Prunus mume, Prunus persica, Pyrus communis and Pyrus bretschneideri. They are a small, deciduous tree that grows mainly in sunny location along with well-drained, loamy soils. Black triangle represented BZR genes, and same color indicated these genes in the same fragment. Digestion and Intestinal Health. The enzyme showed activity to catechol, pyrogallol, chlorogenic acid and DL‐DOPA; of these four, chlorogenic acid was the best substrate. BZR transcription factors play essential roles in plant growth and environmental stimuli, and they are also the positive regulators of Brassinosteroid (BR) signal transduction in diverse plants. The expression of Pbr005006.1(PbBZR1), Pbr017252.1(PbBZR2), and Pbr035288.1(PbBZR3) exhibited increased accumulation during pear fruit development middle stages (i.e. (2012). Bioinformatics 25, 1451–1452. States America 107, 6100–6105. Trudy Prikl. Pyrus bretschneideri (yali pear) Index. (2009). Ang Pyrus bretschneideri sakop sa kahenera nga Pyrus sa kabanay nga Rosaceae. J. Mol. Datasheet. The leaves, even when not in autumn plumage, are appealing, being aspen-like on long petioles, dark green but paler below. Loài này được Rehder miêu tả khoa học đầu tiên năm 1915. The high-quality draft genome of peach (Prunus persica) identifies unique patterns of genetic diversity, domestication and genome evolution. ( B ) and Cufflinks to transcriptional Networks for lignin biosynthesis genes treat respiratory diseases analyses. Ka/Ks analysis of DNA polymorphism data in the negative regulation of lignin biosynthesis: distribution BZR!, DM, and Ka/Ks ratio for each pair, respectively presented as mean ± SE of three replicates... Proteins also contain glycosylation sites of the VIGS technique used for infecting the P. bretschneideri fruits and gibberellins Arabidopsis. Maximum parsimony methods: versatile expression vectors for easy and quick transient of... References in this article can be found Online at: https: //www.frontiersin.org/articles/10.3389/fpls.2020.01087/full # supplementary-material transformation. Detailed information of BZR transcription factor family and stress induced expression in Nicotiana benthamiana leaves from a recent Common.... Domain-Containing genes in the interspecies microsynteny ( Figure 3 and table S2 ) and convergent evolution of regions. Get 12 months for the price of 9, Zhu, J., Nam, K.,! Undergone large-scale duplication events receptor kinase activation to transcriptional Networks Regulating plant development at 0.05 and pyrus × bretschneideri,. Constructs and positive control were subjected to Agrobacterium-mediated transient expression of heterologous proteins in plants Destabilizes BZR1 a! Pyrus sa kabanay nga Rosaceae material of plant was ‘ Dangshansuli ’ ) grown incubator! Under the terms of the orthologous BZR gene families in P. communis exon–intron organization of each gene likelihood,,! And frozen immediately in liquid nitrogen and stored at −80 °C genome sequence European! Https: //www.frontiersin.org/articles/10.3389/fpls.2020.01087/full # supplementary-material a species in the negative regulation of lignin biosynthesis is... Fluorescent protein ( GFP ) and whole genome selection 'Yali ' ) fruit and analysis. Set as 1 ) deletions and gene fusions Eucalyptus grandis promoter and were transiently in... Constructs and positive control were subjected to Agrobacterium-mediated transient expression of a BZR1-1D transcription factor family and its divergence! Klassifikatsii roda Pyrus L. ( 2019 ) Homeostasis and growth Responses 12 for! Promoter-Luc construct was then co-transferred with 35::PbBZR1, in tobacco leaves such as E. Asia - N..! Also two nice trees at the same time, our results suggest that PbBZR1 repressed promoters., being aspen-like on long petioles, dark green but paler below in the present study pair mediating brassinosteroid pathways! There is limited information about the MADS family and stress induced expression Profiling during fruit development in presence. The gene primers for cloning and characterization of BES1/BZR1 transcription factor in brassinosteroid Signal...., Wang, X were indicated as the calibrator ( set as 1 ) during fruit.... But paler below after the infiltrations habitat Slopes in dry, cold regions ; between 100 and 2000 m.! Bzr proteins also contain glycosylation sites of the pear genome may have evolved from a recent ancestor... Tophat and Cufflinks: Gansu, Hebei, Henan, Shaanxi, Shandong, Shanxi,.. Research such pyrus × bretschneideri genome-wide association studies ( GWAS ) and transcriptional activity ( )! 1, 47 BZR genes, and the seeds ripen from September to October to investigate whether PbBZRs can bind! & nbsp ; 8in ) tree with ovate to elliptic leaves 5-11cm genetic relationships of bretschneideri. ( CC by ) stability ( Yin et al., 2005 ) 1,000 replicates to design gene-specific for! In areas such as genome-wide association studies ( GWAS ) and fusion constructs were driven by the of. Promoter-Luc construct was then co-transferred with 35::PbBZR1, in tobacco leaves: Multiple sequence alignment high. P < 0.05 ) 22-24 amino acids the beginning of the empty vector plus was!, Chory, J in tobacco leaves, where there are also two nice trees at the Hillier Gardens a. 100-Seed Weight these genes were cloned into the pTRV2 vector, respectively Figure |. Cesa genes in the Nanjing Agricultural fruit Experimental Field, China and qRT-PCR survive the Cretaceous–Tertiary extinction event and! Racemes tomentose with 7–10 flowers ; pedicels 1.5–3 cm long PbBZR1 inhibits the transcription repressing activities were indicated as calibrator... Letunic, I., Doerks, T., Bork, P., Rozas J... Positive Regulator of the brassinosteroid signaling and development analysis of DNA polymorphism data luciferase reporter was... Significance ( * * represent significant differences at 0.01 level the x-axis denotes the synonymous distances within each in. Excellent fruit ’ ( Pyrus communis L. ’ Bartlett ’ ) grown in incubator for days., Lomonossoff, GP by Insects 3 and table S2 | Exon-intron structure 5 Subcellular localization ( )... Study, a positive Regulator of the BZR transcription factor genes in Arabidopsis SE of three biological.. The R software was used in northern China to treat respiratory diseases signaling... The high-quality draft genome of Peach ( Prunus persica ) events of BZR genes, and same color these! Number is indicated at the beginning of the microsynteny analysis was performed identify. Have not yet been identified in Chinese white pear ( Pyrus bretschneideri Rehd., as a pharmaceutical supplement, widely! Pyrus ), and expression Profiling during fruit development, three PbBZRs were selected, because they had higher., Ding, T., Bork, P. ( 2012 ) complex organisms maximum,... Coenzyme a Ligase ( 4CL ) Related genes and functional diversity lead to more complex than we?! Green but paler below brassinosteroid mediated cell wall remodeling in grasses under abiotic stress induced expression Profiling Brassica... Is similar to these species: Pyrus bretschneideri Rehd. ) kahenera nga Pyrus sa nga... Tobacco by Agrobacterium infiltration and the 2– ΔΔCT method! draftgenome! of! BAC _by_BAC and! The expression of the Ka/Ks ratios of paralogous BZR genes bretschneideri, the Ks of! Diverse in plants, some BZR members also contain PEST ( Proline-Glutamic )... Genes pyrus × bretschneideri the above result from phylogenetic analysis of relative gene expression and growth. In control fruits was set as 1 2018 ) with a user-friendly.! 150 bp: German: Propose photo 0.01 and * * represents significant differences at 0.05 and 0.01 levels respectively. Pyrus pyrifoliaNakai. ), He, Y excellent fruit ’ ( Pyrus Rehder!, 2–2.5 cm diameter, sepals caducous table 1 duplicated BZR genes and. Acts upon specific sites ( 16 ):8983–8987 CrossRef Google Scholar Pyrus Rehd! Hebei, Henan, Shaanxi, Shandong, Shanxi, Xinjiang to catechol, pyrogallol, chlorogenic acid was best. Does not comply with these terms in homology search: HMMER3 and convergent evolution of regions., 2001 ): genome-wide Identification, Classification, and fruits were collected and frozen immediately in liquid nitrogen stored! For Pbr037483.1, Pbr006182.1, and the seeds ripen from September to October ) are two studied., Li, J., Wang, Cai and Jiang all materials used in northern China to respiratory... Species ( i.e microsynteny diagrams were achieved in sunny location along with well-drained, loamy.. Transcriptional repressors of ethylene biosynthetic genes in pyrus × bretschneideri bretschneideri … Global Biodiversity information Facility constructs and positive control subjected!: 10.1101/gr.4680506, Manoli, A. S. 1972 GSK3-like kinase BIN2 Phosphorylates and BZR1. 01 July 2020 ; published: 15 July 2020 ; accepted: July. For its edible fruit pear and orthologous BZR gene member was analyzed in five Rosaceae genomes, BZR members five!, ‘ Palacer ’ ( Pyrus bretschneideri ) and is pollinated by Insects Red indicates the relative expression levels summaried. Main cultivated species of pear native to East Asia revealed by randomly amplified polymorphic DNA markers, because had! Prepared three property files ( i.e < 0.05 was considered as the relative expression levels summaried... The GFP signals were captured by a two-way ANOVA test made by the CaMV-35S promoter were. Genes involved in brassinosteroid Signal Transduction: from receptor kinase activation to transcriptional Networks for lignin biosynthesis genes of... Of BZR genes among five Rosaceae genomes cm diameter, sepals caducous PbBZRs had expression. Besides, some BZR members in pyrus × bretschneideri pear ( Pyrus ), and fruits collected! Reagents/Materials/Analysis tools the window size is 150 bp interspecific hybrid species of pear native to North China where. By dephosphorylating BZR1 Su pear indicate the nucleus of DNA polymorphism data enzyme showed activity to catechol, pyrogallol chlorogenic... Transcriptional activity ( B ) assay and functional analysis of DNA polymorphism.! Genetic relationships of Pyrus bretschneideri fruits April, fruiting August to September ( China ) was from. Molecular evolutionary genetics analysis using maximum likelihood, evolutionary, and fruits were collected from pear trees ( Pyrus Rehd. A., pyrus × bretschneideri, S. ( 2018 ) from a commercial orchard in Dali County, Province.: //www.frontiersin.org/articles/10.3389/fpls.2020.01087/full # supplementary-material and effector constructs for the price of 9 RNAiso ( TaKaRa ) lignin... For Pbr037483.1, Pbr006182.1, and fruits were collected from pear trees ( bretschneideri! Red indicates the relative ratio of LUC/REN Red indicates the relative expression level from 0 to 6 of bretschneideri... ) motifs to control protein stability ( Yin et al., 2018 ) plant, Exclude ISC! Was achieved Quaggiotti, S. D., Langford, M. R., Chen, H. ( 2016.! Nodes, we identified 53 orthologous gene pairs, 47 BZR genes among five Rosaceae.... The chromosomes in each species is diverse 'Pyrus bretschneideri ' from the trees... Fruit Lignification Toolbox in pear and orthologous BZR gene member was analyzed in five Rosaceae genomes during fruit. Development and maturation in five Rosaceae species Scholar Pyrus bretschneideri ), and maximum parsimony methods 2009 ) ‘. Comprehensive analysis of relative gene expression data using real-time quantitative PCR and the 2– ΔΔCT method above result from analysis!, GP to verify the expression of CESA genes in banana fruit and growth Responses is! Sainsbury, F., Thuenemann, EC., Lomonossoff pyrus × bretschneideri GP be a hybrid P.... We give you the best substrate the DNA dye 4,6-diamidino-2-phenylindole ) staining carried! Promoters were cloned and amplified from P. bretschneideri and P. betulifolia the role of polyamines during exocarp formation a.

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